Low pH-induced changes in Pseudomonas exotoxin and its domains: increased binding of Triton X-114
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چکیده
منابع مشابه
Spectroscopic Studies of Triton X-114 and Quercetin/Rutin Solutions
Flavonoids belong to a large group of natural polyphenol compounds and have a broad spectrum of pharmacological activities. Many natural flavonoids are characterized by extremely low solubility in aqueous media and body fluids. To improve the solubility of drugs, various methods were developed and are used in pharmaceutics: solubilization, preparation of solid disperse systems with soluble and ...
متن کاملEffect of low pH on the conformation of Pseudomonas exotoxin A.
Previously we examined factors involved in the entry mechanism of Pseudomonas exotoxin A (PTx) at the level of lipid-protein interactions (Farahbakhsh, Z. T., Baldwin, R. L., and Wisnieski, B. J. (1986) J. Biol. Chem. 261, 11404-11408). Exposure to a low pH environment appears to be an obligatory trigger of the entry pathway. In this report we describe the effect of pH upon the conformation of ...
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A cloud point extraction process using the nonionic surfactant Triton X-114 to extracturanium from aqueous solutions was investigated. The method is based on the complexationreaction of uranium with Arsenazo III and micelle-mediated extraction of the complex. Theoptimal extraction and reaction conditions (e.g., pH, reagent concentration, effect of time) werestudied, and the analytical character...
متن کاملNovel procedure for extraction of a latent grape polyphenoloxidase using temperature-induced phase separation in triton x-114.
Polyphenoloxidase from grape berries is extracted only by nonionic detergents with a hydrophilic-lipophilic balance between 12.4 and 13.5. The enzyme was partially purified in latent form, free of phenolics and chlorophylls, by using temperature phase partitioning in a solution of Triton X-114. This method permits the purification of the enzyme with the same fold purification as the commonly us...
متن کاملPhase separation of integral membrane proteins in Triton X-114 solution.
A solution of the nonionic detergent Triton X-114 is homogeneous at 0 degrees C but separates in an aqueous phase and a detergent phase above 20 degrees C. The extent of this detergent phase separation increases with the temperature and is sensitive to the presence of other surfactants. The partition of proteins during phase separation in solutions of Triton X-114 is investigated. Hydrophilic p...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1990
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.58.5.1415-1420.1990